cd40 knockout (ko) cd45.2 c57bl6 mice Search Results


anti cd40  (Bio X Cell)
96
Bio X Cell anti cd40
Anti Cd40, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd40  (Becton Dickinson)
90
Becton Dickinson cd40
Cd40, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd4  (Becton Dickinson)
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Becton Dickinson cd4
Cd4, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vβ8.3  (Becton Dickinson)
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Becton Dickinson vβ8.3
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mouse antibody anti mouse cd40  (Bio X Cell)
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Bio X Cell mouse antibody anti mouse cd40
Mouse Antibody Anti Mouse Cd40, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ot1  (Jackson Laboratory)
86
Jackson Laboratory ot1
Figure 2. Optimal Tvac frequency and phenotype require the memory fate-associated transcription factor TCF1 WT and TCF1 KO <t>OT1</t> cells were co-transferred into WT <t>C57BL/6</t> recipients that were then vaccinated or challenged with LM-OVA. The phenotype of WT and KO cells 7 days (A–D) or 77 days (E–J) post vaccination/challenge was characterized by flow cytometry. (A) Representative contour plots for CD127 versus TCF1 staining of donor WT and KO cells. (B) The number of total WT and KO OT1 cells split into CD127+ and CD127 subsets. (C) The ratio of the number of WT cells divided by the number of KO cells in (B). (D) The gMFI of CD127 in WT and KO OT1 cells. (E) Representative contour plots for CD127 versus TCF1 staining. (F) The number of total WT and KO OT1 cells split into CD127+ and CD127 subsets. (G) The ratio of the number of WT cells divided by the number of KO cells in (F). (H–J) Representative histograms and gMFIs for CD127 (H), EOMES (I), and FOXO1 (J) in WT and KO OT1 cells after vaccine or LM-OVA challenge. Data shown are mean ± SEM; n R 4 mice per group, representative of two experiments. Significance was defined by unpaired t test with Welch’s correction and two-way ANOVA, where *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
Ot1, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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organisms  (Jackson Laboratory)
86
Jackson Laboratory organisms
Figure 2. Optimal Tvac frequency and phenotype require the memory fate-associated transcription factor TCF1 WT and TCF1 KO <t>OT1</t> cells were co-transferred into WT <t>C57BL/6</t> recipients that were then vaccinated or challenged with LM-OVA. The phenotype of WT and KO cells 7 days (A–D) or 77 days (E–J) post vaccination/challenge was characterized by flow cytometry. (A) Representative contour plots for CD127 versus TCF1 staining of donor WT and KO cells. (B) The number of total WT and KO OT1 cells split into CD127+ and CD127 subsets. (C) The ratio of the number of WT cells divided by the number of KO cells in (B). (D) The gMFI of CD127 in WT and KO OT1 cells. (E) Representative contour plots for CD127 versus TCF1 staining. (F) The number of total WT and KO OT1 cells split into CD127+ and CD127 subsets. (G) The ratio of the number of WT cells divided by the number of KO cells in (F). (H–J) Representative histograms and gMFIs for CD127 (H), EOMES (I), and FOXO1 (J) in WT and KO OT1 cells after vaccine or LM-OVA challenge. Data shown are mean ± SEM; n R 4 mice per group, representative of two experiments. Significance was defined by unpaired t test with Welch’s correction and two-way ANOVA, where *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
Organisms, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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organisms - by Bioz Stars, 2026-10
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dlck cre  (Jackson Laboratory)
86
Jackson Laboratory dlck cre
Figure 2. Optimal Tvac frequency and phenotype require the memory fate-associated transcription factor TCF1 WT and TCF1 KO <t>OT1</t> cells were co-transferred into WT <t>C57BL/6</t> recipients that were then vaccinated or challenged with LM-OVA. The phenotype of WT and KO cells 7 days (A–D) or 77 days (E–J) post vaccination/challenge was characterized by flow cytometry. (A) Representative contour plots for CD127 versus TCF1 staining of donor WT and KO cells. (B) The number of total WT and KO OT1 cells split into CD127+ and CD127 subsets. (C) The ratio of the number of WT cells divided by the number of KO cells in (B). (D) The gMFI of CD127 in WT and KO OT1 cells. (E) Representative contour plots for CD127 versus TCF1 staining. (F) The number of total WT and KO OT1 cells split into CD127+ and CD127 subsets. (G) The ratio of the number of WT cells divided by the number of KO cells in (F). (H–J) Representative histograms and gMFIs for CD127 (H), EOMES (I), and FOXO1 (J) in WT and KO OT1 cells after vaccine or LM-OVA challenge. Data shown are mean ± SEM; n R 4 mice per group, representative of two experiments. Significance was defined by unpaired t test with Welch’s correction and two-way ANOVA, where *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
Dlck Cre, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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acepromazine fatro  (FATRO SpA)
90
FATRO SpA acepromazine fatro
Figure 2. Optimal Tvac frequency and phenotype require the memory fate-associated transcription factor TCF1 WT and TCF1 KO <t>OT1</t> cells were co-transferred into WT <t>C57BL/6</t> recipients that were then vaccinated or challenged with LM-OVA. The phenotype of WT and KO cells 7 days (A–D) or 77 days (E–J) post vaccination/challenge was characterized by flow cytometry. (A) Representative contour plots for CD127 versus TCF1 staining of donor WT and KO cells. (B) The number of total WT and KO OT1 cells split into CD127+ and CD127 subsets. (C) The ratio of the number of WT cells divided by the number of KO cells in (B). (D) The gMFI of CD127 in WT and KO OT1 cells. (E) Representative contour plots for CD127 versus TCF1 staining. (F) The number of total WT and KO OT1 cells split into CD127+ and CD127 subsets. (G) The ratio of the number of WT cells divided by the number of KO cells in (F). (H–J) Representative histograms and gMFIs for CD127 (H), EOMES (I), and FOXO1 (J) in WT and KO OT1 cells after vaccine or LM-OVA challenge. Data shown are mean ± SEM; n R 4 mice per group, representative of two experiments. Significance was defined by unpaired t test with Welch’s correction and two-way ANOVA, where *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
Acepromazine Fatro, supplied by FATRO SpA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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b6 sjl ptprca pepcb boyj mice jackson laboratories strain  (Jackson Laboratory)
86
Jackson Laboratory b6 sjl ptprca pepcb boyj mice jackson laboratories strain
Figure 2. Optimal Tvac frequency and phenotype require the memory fate-associated transcription factor TCF1 WT and TCF1 KO <t>OT1</t> cells were co-transferred into WT <t>C57BL/6</t> recipients that were then vaccinated or challenged with LM-OVA. The phenotype of WT and KO cells 7 days (A–D) or 77 days (E–J) post vaccination/challenge was characterized by flow cytometry. (A) Representative contour plots for CD127 versus TCF1 staining of donor WT and KO cells. (B) The number of total WT and KO OT1 cells split into CD127+ and CD127 subsets. (C) The ratio of the number of WT cells divided by the number of KO cells in (B). (D) The gMFI of CD127 in WT and KO OT1 cells. (E) Representative contour plots for CD127 versus TCF1 staining. (F) The number of total WT and KO OT1 cells split into CD127+ and CD127 subsets. (G) The ratio of the number of WT cells divided by the number of KO cells in (F). (H–J) Representative histograms and gMFIs for CD127 (H), EOMES (I), and FOXO1 (J) in WT and KO OT1 cells after vaccine or LM-OVA challenge. Data shown are mean ± SEM; n R 4 mice per group, representative of two experiments. Significance was defined by unpaired t test with Welch’s correction and two-way ANOVA, where *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
B6 Sjl Ptprca Pepcb Boyj Mice Jackson Laboratories Strain, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chemical compound cfse  (Becton Dickinson)
90
Becton Dickinson chemical compound cfse
Figure 2. Optimal Tvac frequency and phenotype require the memory fate-associated transcription factor TCF1 WT and TCF1 KO <t>OT1</t> cells were co-transferred into WT <t>C57BL/6</t> recipients that were then vaccinated or challenged with LM-OVA. The phenotype of WT and KO cells 7 days (A–D) or 77 days (E–J) post vaccination/challenge was characterized by flow cytometry. (A) Representative contour plots for CD127 versus TCF1 staining of donor WT and KO cells. (B) The number of total WT and KO OT1 cells split into CD127+ and CD127 subsets. (C) The ratio of the number of WT cells divided by the number of KO cells in (B). (D) The gMFI of CD127 in WT and KO OT1 cells. (E) Representative contour plots for CD127 versus TCF1 staining. (F) The number of total WT and KO OT1 cells split into CD127+ and CD127 subsets. (G) The ratio of the number of WT cells divided by the number of KO cells in (F). (H–J) Representative histograms and gMFIs for CD127 (H), EOMES (I), and FOXO1 (J) in WT and KO OT1 cells after vaccine or LM-OVA challenge. Data shown are mean ± SEM; n R 4 mice per group, representative of two experiments. Significance was defined by unpaired t test with Welch’s correction and two-way ANOVA, where *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
Chemical Compound Cfse, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chemical compound violet proliferation dye  (Becton Dickinson)
90
Becton Dickinson chemical compound violet proliferation dye
Figure 2. Optimal Tvac frequency and phenotype require the memory fate-associated transcription factor TCF1 WT and TCF1 KO <t>OT1</t> cells were co-transferred into WT <t>C57BL/6</t> recipients that were then vaccinated or challenged with LM-OVA. The phenotype of WT and KO cells 7 days (A–D) or 77 days (E–J) post vaccination/challenge was characterized by flow cytometry. (A) Representative contour plots for CD127 versus TCF1 staining of donor WT and KO cells. (B) The number of total WT and KO OT1 cells split into CD127+ and CD127 subsets. (C) The ratio of the number of WT cells divided by the number of KO cells in (B). (D) The gMFI of CD127 in WT and KO OT1 cells. (E) Representative contour plots for CD127 versus TCF1 staining. (F) The number of total WT and KO OT1 cells split into CD127+ and CD127 subsets. (G) The ratio of the number of WT cells divided by the number of KO cells in (F). (H–J) Representative histograms and gMFIs for CD127 (H), EOMES (I), and FOXO1 (J) in WT and KO OT1 cells after vaccine or LM-OVA challenge. Data shown are mean ± SEM; n R 4 mice per group, representative of two experiments. Significance was defined by unpaired t test with Welch’s correction and two-way ANOVA, where *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
Chemical Compound Violet Proliferation Dye, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 2. Optimal Tvac frequency and phenotype require the memory fate-associated transcription factor TCF1 WT and TCF1 KO OT1 cells were co-transferred into WT C57BL/6 recipients that were then vaccinated or challenged with LM-OVA. The phenotype of WT and KO cells 7 days (A–D) or 77 days (E–J) post vaccination/challenge was characterized by flow cytometry. (A) Representative contour plots for CD127 versus TCF1 staining of donor WT and KO cells. (B) The number of total WT and KO OT1 cells split into CD127+ and CD127 subsets. (C) The ratio of the number of WT cells divided by the number of KO cells in (B). (D) The gMFI of CD127 in WT and KO OT1 cells. (E) Representative contour plots for CD127 versus TCF1 staining. (F) The number of total WT and KO OT1 cells split into CD127+ and CD127 subsets. (G) The ratio of the number of WT cells divided by the number of KO cells in (F). (H–J) Representative histograms and gMFIs for CD127 (H), EOMES (I), and FOXO1 (J) in WT and KO OT1 cells after vaccine or LM-OVA challenge. Data shown are mean ± SEM; n R 4 mice per group, representative of two experiments. Significance was defined by unpaired t test with Welch’s correction and two-way ANOVA, where *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Journal: Cell reports

Article Title: Vaccine adjuvant-elicited CD8 + T cell immunity is co-dependent on T-bet and FOXO1.

doi: 10.1016/j.celrep.2023.112911

Figure Lengend Snippet: Figure 2. Optimal Tvac frequency and phenotype require the memory fate-associated transcription factor TCF1 WT and TCF1 KO OT1 cells were co-transferred into WT C57BL/6 recipients that were then vaccinated or challenged with LM-OVA. The phenotype of WT and KO cells 7 days (A–D) or 77 days (E–J) post vaccination/challenge was characterized by flow cytometry. (A) Representative contour plots for CD127 versus TCF1 staining of donor WT and KO cells. (B) The number of total WT and KO OT1 cells split into CD127+ and CD127 subsets. (C) The ratio of the number of WT cells divided by the number of KO cells in (B). (D) The gMFI of CD127 in WT and KO OT1 cells. (E) Representative contour plots for CD127 versus TCF1 staining. (F) The number of total WT and KO OT1 cells split into CD127+ and CD127 subsets. (G) The ratio of the number of WT cells divided by the number of KO cells in (F). (H–J) Representative histograms and gMFIs for CD127 (H), EOMES (I), and FOXO1 (J) in WT and KO OT1 cells after vaccine or LM-OVA challenge. Data shown are mean ± SEM; n R 4 mice per group, representative of two experiments. Significance was defined by unpaired t test with Welch’s correction and two-way ANOVA, where *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies aCD16/32 hybridoma supernatent 24G2 anti-CD19 Biolegend clone 6D5 anti-CD8a Biolegend clone 53–6.7 anti-CD44 Tonbo clone IM-7 anti-CD127 Tonbo clone A7R34 anti-KLRG1 Biolegend clone 2F1/KLRG1 anti-CD45.1 Biolegend Clone A20 anti-CD45.2 Biolegend clone 104 anti-CD122 Biolegend clone TM-b1 anti-FOXO1 Cell Signaling Technology clone C29H4 anti-Tbet Biolegend clone 4B10 anti-TCF1 Cell Signaling Technology clone C63D9 anti-Eomes Biolegend clone Dan11mag anti-CD40 BioXcell clone FGK4.5 Bacterial and virus strains ova-expressing Listeria monocytogenes Hao Shen LM-OVA Chemicals, peptides, and recombinant proteins Kb-SIINFEKL tetramer NIH Tetramer core facility -PE or -APC conjugated Fix/Perm solution Tonbo Cat# TNB1022L160 Flow Cytometry Perm Buffer Tonbo Cat# TNB-1213-L150 poly(I:C) InvivoGen cat# tlrl-pic CpG ODN 1826 InvivoGen Cat# tlrl-1826 monophosphoryl lipid A (MPLA) InvivoGen cat# vac-mpla2 Pam3Cys InvivoGen Cat# tlrl-pms Alu-Gel-S SERVA Electrophoresis GmbH Cat# 12261.01 Ovalbumin Millipore-Sigma Cat# A5503 Neuro9 lipid mix Precision Nanosystem catalog#: NWS0001 Deposited data scRNA data University of Colorado Cancer Center Genomics and Microarray Core Facility GSE237416 RNAseq data University of Colorado Cancer Center Genomics and Microarray Core Facility GSE237415 Experimental models: Organisms/strains C57BL/6J mice Jackson Laboratories Strain #000664 B6.SJL-Ptprca Pepcb/BoyJ mice Jackson Laboratories Strain #:002014 OT1 (C57BL/6-Tg(TcraTcrb)1100Mjb/J) Jackson Laboratories Strain #:003831 dLck-Cre (B6.Cg-Tg(Lck-icre)3779Nik/J) Jackson Laboratories Strain #012837 Tcf7flox/flox 3 CD4-Cre Tuoqi Wu UT Southwestern Foxo1flox/flox (Foxo1tmRdp) Stephen Hedrick UCSD Tbx21flox/flox (B6.129-Tbx21tm2Srnr/J) Christopher Hunter U. Penn (Continued on next page) 16 Cell Reports 42, 112911, August 29, 2023

Techniques: Cytometry, Staining

Figure 3. Tvacs require FOXO1 for peak and memory responses WT and FOXO1 KO OT1 cells were co-transferred into WT C57BL/6 recipients that were then vacci- nated or challenged with LM-OVA. On day 7 post challenge, the splenic WT and KO OT1 phenotype was analyzed by flow cytometry. (A) Representative contour plots for CD127 versus TCF1 staining. (B) Representative contour plots for TCF1 versus FOXO1 staining. (C) The number of total WT and KO OT1 cells split into CD127+ and CD127 subsets. (D) The number of WT cells divided by the number of KO cells normalized to input. (E) The percentage of TCF1+ in WT and KO OT1 cells. Data shown are mean ± SEM; n R 4 mice per group, representative of two experiments. Signifi- cance was defined by unpaired t test with Welch’s correction and two-way ANOVA, where *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Journal: Cell reports

Article Title: Vaccine adjuvant-elicited CD8 + T cell immunity is co-dependent on T-bet and FOXO1.

doi: 10.1016/j.celrep.2023.112911

Figure Lengend Snippet: Figure 3. Tvacs require FOXO1 for peak and memory responses WT and FOXO1 KO OT1 cells were co-transferred into WT C57BL/6 recipients that were then vacci- nated or challenged with LM-OVA. On day 7 post challenge, the splenic WT and KO OT1 phenotype was analyzed by flow cytometry. (A) Representative contour plots for CD127 versus TCF1 staining. (B) Representative contour plots for TCF1 versus FOXO1 staining. (C) The number of total WT and KO OT1 cells split into CD127+ and CD127 subsets. (D) The number of WT cells divided by the number of KO cells normalized to input. (E) The percentage of TCF1+ in WT and KO OT1 cells. Data shown are mean ± SEM; n R 4 mice per group, representative of two experiments. Signifi- cance was defined by unpaired t test with Welch’s correction and two-way ANOVA, where *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies aCD16/32 hybridoma supernatent 24G2 anti-CD19 Biolegend clone 6D5 anti-CD8a Biolegend clone 53–6.7 anti-CD44 Tonbo clone IM-7 anti-CD127 Tonbo clone A7R34 anti-KLRG1 Biolegend clone 2F1/KLRG1 anti-CD45.1 Biolegend Clone A20 anti-CD45.2 Biolegend clone 104 anti-CD122 Biolegend clone TM-b1 anti-FOXO1 Cell Signaling Technology clone C29H4 anti-Tbet Biolegend clone 4B10 anti-TCF1 Cell Signaling Technology clone C63D9 anti-Eomes Biolegend clone Dan11mag anti-CD40 BioXcell clone FGK4.5 Bacterial and virus strains ova-expressing Listeria monocytogenes Hao Shen LM-OVA Chemicals, peptides, and recombinant proteins Kb-SIINFEKL tetramer NIH Tetramer core facility -PE or -APC conjugated Fix/Perm solution Tonbo Cat# TNB1022L160 Flow Cytometry Perm Buffer Tonbo Cat# TNB-1213-L150 poly(I:C) InvivoGen cat# tlrl-pic CpG ODN 1826 InvivoGen Cat# tlrl-1826 monophosphoryl lipid A (MPLA) InvivoGen cat# vac-mpla2 Pam3Cys InvivoGen Cat# tlrl-pms Alu-Gel-S SERVA Electrophoresis GmbH Cat# 12261.01 Ovalbumin Millipore-Sigma Cat# A5503 Neuro9 lipid mix Precision Nanosystem catalog#: NWS0001 Deposited data scRNA data University of Colorado Cancer Center Genomics and Microarray Core Facility GSE237416 RNAseq data University of Colorado Cancer Center Genomics and Microarray Core Facility GSE237415 Experimental models: Organisms/strains C57BL/6J mice Jackson Laboratories Strain #000664 B6.SJL-Ptprca Pepcb/BoyJ mice Jackson Laboratories Strain #:002014 OT1 (C57BL/6-Tg(TcraTcrb)1100Mjb/J) Jackson Laboratories Strain #:003831 dLck-Cre (B6.Cg-Tg(Lck-icre)3779Nik/J) Jackson Laboratories Strain #012837 Tcf7flox/flox 3 CD4-Cre Tuoqi Wu UT Southwestern Foxo1flox/flox (Foxo1tmRdp) Stephen Hedrick UCSD Tbx21flox/flox (B6.129-Tbx21tm2Srnr/J) Christopher Hunter U. Penn (Continued on next page) 16 Cell Reports 42, 112911, August 29, 2023

Techniques: Cytometry, Staining

Figure 4. Tvac peak and memory responses are compromised in the absence of T-bet WT and T-bet KO OT1 cells were co-transferred into WT C57BL/6 recipients that were then vaccinated or challenged with LM-OVA. On day 7 (A–E) or 50 (F–J) post challenge, the splenic WT and KO OT1 phenotype was analyzed by flow cytometry. (A) Representative contour plots for CD127 versus TCF1 expression in WT and KO OT1 cells. (B) The number of WT and KO OT1 cells split into CD127+ and CD127 subsets. (C) The ratio of WT:KO CD127+ cells. (D) Representative histograms for FOXO1 staining of WT and KO OT1 cells. (E) FOXO1 gMFI of WT and KO OT1 cells. (F) Representative contour plots for CD127 versus TCF1 expression in WT and KO cells on day 50 post vaccination and infection. (G) The number of WT and KO OT1 cells split into CD127+ and CD127 subsets. (H) The ratio of WT:KO OT1 cells. (I) Representative histograms for FOXO1 staining of WT and KO OT1 cells.

Journal: Cell reports

Article Title: Vaccine adjuvant-elicited CD8 + T cell immunity is co-dependent on T-bet and FOXO1.

doi: 10.1016/j.celrep.2023.112911

Figure Lengend Snippet: Figure 4. Tvac peak and memory responses are compromised in the absence of T-bet WT and T-bet KO OT1 cells were co-transferred into WT C57BL/6 recipients that were then vaccinated or challenged with LM-OVA. On day 7 (A–E) or 50 (F–J) post challenge, the splenic WT and KO OT1 phenotype was analyzed by flow cytometry. (A) Representative contour plots for CD127 versus TCF1 expression in WT and KO OT1 cells. (B) The number of WT and KO OT1 cells split into CD127+ and CD127 subsets. (C) The ratio of WT:KO CD127+ cells. (D) Representative histograms for FOXO1 staining of WT and KO OT1 cells. (E) FOXO1 gMFI of WT and KO OT1 cells. (F) Representative contour plots for CD127 versus TCF1 expression in WT and KO cells on day 50 post vaccination and infection. (G) The number of WT and KO OT1 cells split into CD127+ and CD127 subsets. (H) The ratio of WT:KO OT1 cells. (I) Representative histograms for FOXO1 staining of WT and KO OT1 cells.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies aCD16/32 hybridoma supernatent 24G2 anti-CD19 Biolegend clone 6D5 anti-CD8a Biolegend clone 53–6.7 anti-CD44 Tonbo clone IM-7 anti-CD127 Tonbo clone A7R34 anti-KLRG1 Biolegend clone 2F1/KLRG1 anti-CD45.1 Biolegend Clone A20 anti-CD45.2 Biolegend clone 104 anti-CD122 Biolegend clone TM-b1 anti-FOXO1 Cell Signaling Technology clone C29H4 anti-Tbet Biolegend clone 4B10 anti-TCF1 Cell Signaling Technology clone C63D9 anti-Eomes Biolegend clone Dan11mag anti-CD40 BioXcell clone FGK4.5 Bacterial and virus strains ova-expressing Listeria monocytogenes Hao Shen LM-OVA Chemicals, peptides, and recombinant proteins Kb-SIINFEKL tetramer NIH Tetramer core facility -PE or -APC conjugated Fix/Perm solution Tonbo Cat# TNB1022L160 Flow Cytometry Perm Buffer Tonbo Cat# TNB-1213-L150 poly(I:C) InvivoGen cat# tlrl-pic CpG ODN 1826 InvivoGen Cat# tlrl-1826 monophosphoryl lipid A (MPLA) InvivoGen cat# vac-mpla2 Pam3Cys InvivoGen Cat# tlrl-pms Alu-Gel-S SERVA Electrophoresis GmbH Cat# 12261.01 Ovalbumin Millipore-Sigma Cat# A5503 Neuro9 lipid mix Precision Nanosystem catalog#: NWS0001 Deposited data scRNA data University of Colorado Cancer Center Genomics and Microarray Core Facility GSE237416 RNAseq data University of Colorado Cancer Center Genomics and Microarray Core Facility GSE237415 Experimental models: Organisms/strains C57BL/6J mice Jackson Laboratories Strain #000664 B6.SJL-Ptprca Pepcb/BoyJ mice Jackson Laboratories Strain #:002014 OT1 (C57BL/6-Tg(TcraTcrb)1100Mjb/J) Jackson Laboratories Strain #:003831 dLck-Cre (B6.Cg-Tg(Lck-icre)3779Nik/J) Jackson Laboratories Strain #012837 Tcf7flox/flox 3 CD4-Cre Tuoqi Wu UT Southwestern Foxo1flox/flox (Foxo1tmRdp) Stephen Hedrick UCSD Tbx21flox/flox (B6.129-Tbx21tm2Srnr/J) Christopher Hunter U. Penn (Continued on next page) 16 Cell Reports 42, 112911, August 29, 2023

Techniques: Cytometry, Expressing, Staining, Infection

Figure 6. Adjuvant-elicited T cells express dual effector and memory transcriptional programs (A) Uniform Manifold Approximation and Projection (UMAP) Dimensionality reduction mapping analysis of single-cell gene expression data of OT1 cells isolated 7 days post infection with LM-OVA (LM, purple) or adjuvanted OVA vaccine (Adj, black). (B) UMAP as in (A), with cells color coded by clusters derived from shared nearest neighbor analysis. (C) Proportion of either Adj- or LM-elicited T cells within clusters depicted in (B). (D) UMAP as in (A), with cells color coded by cell cycle status inferred from transcriptional profiles. (E) Violin plots depicting expression of select genes associated with either the effector (left) or memory (right) phenotype within clusters 1 and 2. (F) Violin plots depicting the module score of gene sets associated with effector (left) or memory (right) within clusters 1 and 2. (G) Violin plots of SCENIC-inferred transcription factor activity within clusters 1 and 2.

Journal: Cell reports

Article Title: Vaccine adjuvant-elicited CD8 + T cell immunity is co-dependent on T-bet and FOXO1.

doi: 10.1016/j.celrep.2023.112911

Figure Lengend Snippet: Figure 6. Adjuvant-elicited T cells express dual effector and memory transcriptional programs (A) Uniform Manifold Approximation and Projection (UMAP) Dimensionality reduction mapping analysis of single-cell gene expression data of OT1 cells isolated 7 days post infection with LM-OVA (LM, purple) or adjuvanted OVA vaccine (Adj, black). (B) UMAP as in (A), with cells color coded by clusters derived from shared nearest neighbor analysis. (C) Proportion of either Adj- or LM-elicited T cells within clusters depicted in (B). (D) UMAP as in (A), with cells color coded by cell cycle status inferred from transcriptional profiles. (E) Violin plots depicting expression of select genes associated with either the effector (left) or memory (right) phenotype within clusters 1 and 2. (F) Violin plots depicting the module score of gene sets associated with effector (left) or memory (right) within clusters 1 and 2. (G) Violin plots of SCENIC-inferred transcription factor activity within clusters 1 and 2.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies aCD16/32 hybridoma supernatent 24G2 anti-CD19 Biolegend clone 6D5 anti-CD8a Biolegend clone 53–6.7 anti-CD44 Tonbo clone IM-7 anti-CD127 Tonbo clone A7R34 anti-KLRG1 Biolegend clone 2F1/KLRG1 anti-CD45.1 Biolegend Clone A20 anti-CD45.2 Biolegend clone 104 anti-CD122 Biolegend clone TM-b1 anti-FOXO1 Cell Signaling Technology clone C29H4 anti-Tbet Biolegend clone 4B10 anti-TCF1 Cell Signaling Technology clone C63D9 anti-Eomes Biolegend clone Dan11mag anti-CD40 BioXcell clone FGK4.5 Bacterial and virus strains ova-expressing Listeria monocytogenes Hao Shen LM-OVA Chemicals, peptides, and recombinant proteins Kb-SIINFEKL tetramer NIH Tetramer core facility -PE or -APC conjugated Fix/Perm solution Tonbo Cat# TNB1022L160 Flow Cytometry Perm Buffer Tonbo Cat# TNB-1213-L150 poly(I:C) InvivoGen cat# tlrl-pic CpG ODN 1826 InvivoGen Cat# tlrl-1826 monophosphoryl lipid A (MPLA) InvivoGen cat# vac-mpla2 Pam3Cys InvivoGen Cat# tlrl-pms Alu-Gel-S SERVA Electrophoresis GmbH Cat# 12261.01 Ovalbumin Millipore-Sigma Cat# A5503 Neuro9 lipid mix Precision Nanosystem catalog#: NWS0001 Deposited data scRNA data University of Colorado Cancer Center Genomics and Microarray Core Facility GSE237416 RNAseq data University of Colorado Cancer Center Genomics and Microarray Core Facility GSE237415 Experimental models: Organisms/strains C57BL/6J mice Jackson Laboratories Strain #000664 B6.SJL-Ptprca Pepcb/BoyJ mice Jackson Laboratories Strain #:002014 OT1 (C57BL/6-Tg(TcraTcrb)1100Mjb/J) Jackson Laboratories Strain #:003831 dLck-Cre (B6.Cg-Tg(Lck-icre)3779Nik/J) Jackson Laboratories Strain #012837 Tcf7flox/flox 3 CD4-Cre Tuoqi Wu UT Southwestern Foxo1flox/flox (Foxo1tmRdp) Stephen Hedrick UCSD Tbx21flox/flox (B6.129-Tbx21tm2Srnr/J) Christopher Hunter U. Penn (Continued on next page) 16 Cell Reports 42, 112911, August 29, 2023

Techniques: Adjuvant, Gene Expression, Isolation, Infection, Derivative Assay, Expressing, Activity Assay

Figure 7. CD8 responses to mRNA-LNP vaccination are also T-bet and FOXO1 dependent WT (CD45.1/1) and KO (CD45.1/2) OT1 cells were co-transferred into CD45.2/2 C57BL/6 recipients. One day later, the mice were vaccinated with OVA-encoding mRNA-LNPs as described in the STAR Methods. On day 10 post vaccination, the spleens were harvested, and the WT:KO ratio and phenotype of the responding OT1s were was analyzed by flow cytometry. (A) Representative dot plots of spleen cells, gated only on the transferred T cells, in mice co-transferred with WT:FOXO KO (left) or WT:T-bet KO (right) cells. Numbers represent percentages of WT or KO T cells of total transferred T cells in each panel. (B) Quantification of WT and KO T cell numbers from (A). (C) Ratio of WT:KO T cells in each recipient. Data shown are mean ± SEM; representative of two experiments with n = 8–10 mice per group. Significance was defined by paired t test, where *p < 0.05, **p < 0.01.

Journal: Cell reports

Article Title: Vaccine adjuvant-elicited CD8 + T cell immunity is co-dependent on T-bet and FOXO1.

doi: 10.1016/j.celrep.2023.112911

Figure Lengend Snippet: Figure 7. CD8 responses to mRNA-LNP vaccination are also T-bet and FOXO1 dependent WT (CD45.1/1) and KO (CD45.1/2) OT1 cells were co-transferred into CD45.2/2 C57BL/6 recipients. One day later, the mice were vaccinated with OVA-encoding mRNA-LNPs as described in the STAR Methods. On day 10 post vaccination, the spleens were harvested, and the WT:KO ratio and phenotype of the responding OT1s were was analyzed by flow cytometry. (A) Representative dot plots of spleen cells, gated only on the transferred T cells, in mice co-transferred with WT:FOXO KO (left) or WT:T-bet KO (right) cells. Numbers represent percentages of WT or KO T cells of total transferred T cells in each panel. (B) Quantification of WT and KO T cell numbers from (A). (C) Ratio of WT:KO T cells in each recipient. Data shown are mean ± SEM; representative of two experiments with n = 8–10 mice per group. Significance was defined by paired t test, where *p < 0.05, **p < 0.01.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies aCD16/32 hybridoma supernatent 24G2 anti-CD19 Biolegend clone 6D5 anti-CD8a Biolegend clone 53–6.7 anti-CD44 Tonbo clone IM-7 anti-CD127 Tonbo clone A7R34 anti-KLRG1 Biolegend clone 2F1/KLRG1 anti-CD45.1 Biolegend Clone A20 anti-CD45.2 Biolegend clone 104 anti-CD122 Biolegend clone TM-b1 anti-FOXO1 Cell Signaling Technology clone C29H4 anti-Tbet Biolegend clone 4B10 anti-TCF1 Cell Signaling Technology clone C63D9 anti-Eomes Biolegend clone Dan11mag anti-CD40 BioXcell clone FGK4.5 Bacterial and virus strains ova-expressing Listeria monocytogenes Hao Shen LM-OVA Chemicals, peptides, and recombinant proteins Kb-SIINFEKL tetramer NIH Tetramer core facility -PE or -APC conjugated Fix/Perm solution Tonbo Cat# TNB1022L160 Flow Cytometry Perm Buffer Tonbo Cat# TNB-1213-L150 poly(I:C) InvivoGen cat# tlrl-pic CpG ODN 1826 InvivoGen Cat# tlrl-1826 monophosphoryl lipid A (MPLA) InvivoGen cat# vac-mpla2 Pam3Cys InvivoGen Cat# tlrl-pms Alu-Gel-S SERVA Electrophoresis GmbH Cat# 12261.01 Ovalbumin Millipore-Sigma Cat# A5503 Neuro9 lipid mix Precision Nanosystem catalog#: NWS0001 Deposited data scRNA data University of Colorado Cancer Center Genomics and Microarray Core Facility GSE237416 RNAseq data University of Colorado Cancer Center Genomics and Microarray Core Facility GSE237415 Experimental models: Organisms/strains C57BL/6J mice Jackson Laboratories Strain #000664 B6.SJL-Ptprca Pepcb/BoyJ mice Jackson Laboratories Strain #:002014 OT1 (C57BL/6-Tg(TcraTcrb)1100Mjb/J) Jackson Laboratories Strain #:003831 dLck-Cre (B6.Cg-Tg(Lck-icre)3779Nik/J) Jackson Laboratories Strain #012837 Tcf7flox/flox 3 CD4-Cre Tuoqi Wu UT Southwestern Foxo1flox/flox (Foxo1tmRdp) Stephen Hedrick UCSD Tbx21flox/flox (B6.129-Tbx21tm2Srnr/J) Christopher Hunter U. Penn (Continued on next page) 16 Cell Reports 42, 112911, August 29, 2023

Techniques: Cytometry